Recombinant Protein Stability in Cyanobacteria

Enhancing recombinant protein quality and yield by protein stability profiling.

The reliable production of large amounts of stable, high-quality proteins is a major challenge facing pharmaceutical protein biochemists, necessary for fulfilling demands from structural biology, for high-throughput screening, and for assay purposes throughout early discovery. One strategy for bypassing purification challenges in problematic systems is to engineer multiple forms of a particular...

Advances and challenges in recombinant protein expression in Escherichia coli

Mobility of pigment - protein complexes in Cyanobacteria

Phycobilisomes, the light harvesting complexes of cyanobacteria are highly mobile, fluorescent complexes known to diffuse freely on the thylakoid membranes, interacting with the reaction centre complexes to mediate efficient photosynthesis. The primary aim of this project is to establish what processes require this rapid movement of the complexes using a number of genetic, biochemical and micro...

Stress response in cyanobacteria

Cyanobacteria are an important source of natural products. In this article, we briefly review the responses of cyanobacteria to different stresses. Abiotic stresses (temperature, salt, heavy metals, metalloid and ultraviolet (UV) influence cell growth and metabolism in cyanobacteria. Salt stress is a major abiotic factor that decrease...

Plasmid stability in recombinant Saccharomyces cerevisiae.

Because of many advantages, the yeast Saccharomyces cerevisiae is increasingly being employed for expression of recombinant proteins. Usually, hybrid plasmids (shuttle vectors) are employed as carriers to introduce the foreign DNA into the yeast host. Unfortunately, the transformed host often suffers from some kind of instability, tending to lose or alter the foreign plasmid. Construction of st...